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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal logo EmBarc

European Microbial DNA Bank Network


DNA extraction, storage and quality control

EMbaRC Biological Resource Centres of micro-organisms are glad to provide their internal procedures for DNA extraction, storage and quality control.

Procedures available for download.

Biological Resource Centre

Main resources preserved


Procedure for DNA extraction

Procedure for DNA storage

Procedure for Quality control

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Filamentous fungi

logo CABI


Filamentous fungi

United Kingdom

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Filamentous fungi and Yeasts

the Netherlands

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Bacteria, Filamentous fungi and Yeasts


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Food bacteria



Pathogenic bacteria


Plant-associated bacteria


Filamentous fungi



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Bacteria and Archaea


logo Pasteur Institute




logo MUM


Filamentous fungi


Comments on extraction methods

Four extraction methods are proposed by CABI:

  • DNA extraction from Bacterial and Yeast material
  • DNA extraction from Fungal material (excluding Yeasts) using the commercial formulation microLYSIS®-PLUS
  • DNA extraction from Fungal material using the commercial formulation DNAMITE® for difficult cells
  • DNA extraction from Fungal material using the commercial formulation DNeasy® Plant kit

Two extraction methods are proposed by CIRM-BIA:

  • DNA preparation with the kit Nucleospin® tissue from Macherey Nagel, using a high throughput screening robot
  • DNA preparation with the kit DNeasy® Blood&Tissue Kit, Qiagen (manual method)

Four extraction methods are proposed by CBS:

  • DNA isolation with Bio 101® FastDNA Kit
  • DNA isolation with MoBio Microbial DNA Isolation Kit
  • DNA isolation with SDS and CTAB based lysis and chloroform purification
  • DNA isolation with SDS based lysis and phenol chloroform purification

Comments on Quality control methods

Two Quality control methods are proposed by CABI:

  • DNA Quality control by DNA fingerprinting
  • DNA Quality control by Gel electrophoresis