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Last update: May 2021

Menu Logo Principal logo EmBarc

European Microbial DNA Bank Network

Procedures

DNA extraction, storage and quality control

EMbaRC Biological Resource Centres of micro-organisms are glad to provide their internal procedures for DNA extraction, storage and quality control.

Procedures available for download.

Biological Resource Centre

Main resources preserved

Country

Procedure for DNA extraction

Procedure for DNA storage

Procedure for Quality control

logo BCCM

BCCM/LMBP

Plasmids

Belgium

BCCM/LMG

Bacteria

BCCM/MUCL

Filamentous fungi

logo CABI

CABI

Filamentous fungi

United Kingdom

logo CBS

CBS

Filamentous fungi and Yeasts

the Netherlands

logo CECT

CECT

Bacteria, Filamentous fungi and Yeasts

Spain

logo CIRM

CIRM-BIA

Food bacteria

France

CIRM-BP

Pathogenic bacteria

CIRM-CFBP

Plant-associated bacteria

CIRM-CF

Filamentous fungi

CIRM-Levures

Yeasts

logo DSMZ

DSMZ

Bacteria and Archaea

Germany

logo Pasteur Institute

CRBIP

Bacteria

France

logo MUM

MUM

Filamentous fungi

Portugal

Comments on extraction methods

Four extraction methods are proposed by CABI:

  • DNA extraction from Bacterial and Yeast material
  • DNA extraction from Fungal material (excluding Yeasts) using the commercial formulation microLYSIS®-PLUS
  • DNA extraction from Fungal material using the commercial formulation DNAMITE® for difficult cells
  • DNA extraction from Fungal material using the commercial formulation DNeasy® Plant kit

Two extraction methods are proposed by CIRM-BIA:

  • DNA preparation with the kit Nucleospin® tissue from Macherey Nagel, using a high throughput screening robot
  • DNA preparation with the kit DNeasy® Blood&Tissue Kit, Qiagen (manual method)

Four extraction methods are proposed by CBS:

  • DNA isolation with Bio 101® FastDNA Kit
  • DNA isolation with MoBio Microbial DNA Isolation Kit
  • DNA isolation with SDS and CTAB based lysis and chloroform purification
  • DNA isolation with SDS based lysis and phenol chloroform purification

Comments on Quality control methods

Two Quality control methods are proposed by CABI:

  • DNA Quality control by DNA fingerprinting
  • DNA Quality control by Gel electrophoresis